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下列关于探针标记方法的叙述正确的是

单选题
2022-04-02 11:46
A、酶促标记法是最常用的标记方法,产生比化学标记法高的敏感性
B、酶促标记法简便、快速、标记均匀
C、末端标记的探针比均匀标记的探针有更高的活性
D、末端标记的探针常用于杂交分析
E、均匀标记的探针主要用于DNA的测序
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正确答案
A

试题解析
酶促标记法是最常用的标记方法,可更好地修饰DNA,产生比化学标记法高的敏感性,其缺点是操作较复杂、产量低、成本高。化学标记法简便、快速、标记均勾。从这两大类标记方法已分别衍生出多种标记方法,应根据不同的需要进行选择,各种标记方法最终分别产生均匀标记的探针或末端标记的探针。均匀标记的探针由于每个核酸分子中掺入很多标记的dNTP,因而比末端标记的探针有更高的活性。均匀标记的探针常用于杂交分析,而末端标记的探针主要用于DNA测序。

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