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Northern印迹与Southern印迹有什么不同?

问答题
2022-06-20 23:27
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正确答案

Northern印迹的原理同Southern印迹相比有两点不同:
(1)转移的对象不同,Northern印迹是将RNA变性及电泳分离后,将其转移到固相支持物上的过程。
(2)虽然RNA电泳前不需像DNA那样进行酶切,但也需要变性。不过变性方法是不同的,它不能用碱变性,只是在加样前用甲醛、乙二醛或甲基氢氧化银变性,因为碱变性会导致RNA的降解。


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