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简述革兰氏染色的过程。

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2021-07-18 00:01
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(1)在无菌条件下,用接种环挑取少量细菌于干净的载玻片上涂布均匀,固定。(1分)(2)用草酸铵洁净紫染色1min,水洗去掉浮色。(1分)(3)用碘-碘化钾溶液媒染1min,倾去多余溶液。(1分)(4)乙醇脱色,革兰氏阳性菌不被退色而呈紫色,革兰氏阴性菌呈无色。(1分)(5)用番红染液复染1min,革兰氏阳性菌仍呈紫色,革兰氏阴性菌呈红色。(1分)

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