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fluorescence resonance energy transfer (荧光共振能量转移)

名词解释题
2021-09-02 20:40
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当一个荧光分子(又称为供体分子)的荧光光谱与另一个荧光分子(又称为受体分子)的激发光谱相重叠时,供体荧光分子的激发能诱发受体分子发出荧光,同时供体荧光分子自身的荧光强度衰减。FRET程度与供、受体分子的空间距离紧密相关,一般为7~10nm时即可发生FRET;随着距离延长,FRET呈显著减弱。


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