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关于聚合酶链反应（PCR）引物的叙述正确的是

单选题

2022-04-02 11:46

A、引物是一条人工合成的寡核苷酸片段
B、引物序列与模板DNA的待扩增区互补
C、在已知序列的模板DNA待扩增区两端各有一条引物
D、引物自身应该存在互补序列
E、引物的3＇端可以被修饰

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正确答案

试题解析

进行PCR扩增的首要条件是设计一对人工合成的寡核苷酸引物。该引物序列与待扩增DNA模板两端的碱基序列互补。引物设计的原则包括：①引物长度15~30个碱基，不能超过38个；②G+C含量一般为40%~60%；③碱基分布随机，避免数个嘌呤或嘧啶的连续排列；④引物自身不应存在互补序列；⑤两个引物之间不应有多于4个的互补序列；⑥引物的3＇端不应有任何修饰，5＇端则可以被修饰；⑦引物应有特异性。

标签：川北医学院药学专升本分子生物学作业4

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