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PCR技术的基本原理

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2022-06-02 00:08
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正确答案

该技术是在模板DNA、引物和四种脱氧核糖核苷酸存在下,依赖于DNA聚合酶的酶促合成反应。
DNA聚合酶以单链DNA为模板,借助一小段双链DNA来启动合成,通过一个或两个人工合成的寡核苷酸引物与单链DNA模板中的一段互补序列结合,形成部分双链。
在适宜的温度和环境下,DNA聚合酶将脱氧单核苷酸加到引物3´-OH末端,并以此为起始点,沿模板5´→3´方向延伸,合成一条新的DNA互补链


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