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关于关于重组PCR定点突变法叙述正确的是()

多选题
2022-03-01 23:28
A、增加镁离子浓度容易产生正向突变
B、第一轮PCR扩增的是不同DNA模版链
C、第二轮PCR扩增的是同一DNA模板链
D、第三轮PCR需要两种外侧寡核苷酸引物进行扩增
E、该方法产生的DNA片段需要经过测序来验证是否发生其他突变
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正确答案
B | D

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